Efficient In Vitro Regeneration, Analysis of Molecular Fidelity and Agrobacterium tumifaciens - Mediated Genetic Transformation of Grewia asiatica L.
نویسندگان
چکیده
منابع مشابه
Regeneration and Agrobacterium-mediated transformation of three economically important strawberry cultivars Kurdistan, Camarosa and Paros
Genetic transformation studies were carried out to standardize a protocol for Agrobacterium -mediated genetic transformation of three economically important strawberry (Fragaria x ananassa Duch) cultivars Kurdistan’, Camarosa’, and ‘Paros’. Shoot regeneration frequency 72, 65 and 30% was obtained on MS (1) basal medium supplemented with 2% glucose and 4 mg/l TDZ for Camarosa, Kurdistan and Paro...
متن کاملAgrobacterium Mediated Transformation of Maize (Zea mays L.)
Agrobacterium tumefaciens mediated transformation may offer a better alternative than the biolistic gun for genetic transformation of maize plants. This gene delivery system results in a greater proportion of stable, low-copy number transgenic events than does the biolistic gun, and is highly efficient. In the present work, we studied maize transformation using A. tumefaciens by identifying som...
متن کاملHigh frequency regeneration via direct somatic embryogenesis and efficient Agrobacterium- mediated genetic transformation of tobacco
A direct somatic embryogenesis protocol was developed for four cultivars of Nicotiana species, by using leaf disc as an explant. Direct somatic embryogenesis of Nicotiana by using BAP and IAA has not been investigated so far. This method does not require formation of callus tissues which leads to somaclonal variations. The frequency of somatic embryogenesis was strongly influenced by the plant ...
متن کاملEfficient Agrobacterium-Mediated Transformation and Analysis of Transgenic Plants in Hybrid Black Poplar (Populus × euromericana Dode Guinier)
Black poplar (Populus× euramericana Dode Guinier) is an industrially important tree with broad applications in wood and paper, biofuel and cellulose-based industries as well as plant breeding programs and soil phytoremediation approaches. Here, we have focused on development of direct shoot regeneration and Agrobacterium-mediated transformation protocols using the in vitro internodal stem tissu...
متن کاملAgrobacterium Mediated Genetic Transformation of Eggplant (Solanum Melongena L.)
An efficient variety-independent method for producing transgenic eggplants (Solanum melongena L.) via Agrobacterium tumefaciens-mediated genetic transformation was developed. Two weeks old cotyledon explants were transformed by co-cultivation with Agrobacterium tumefaciens strain LBA4404 harboring a binary vector pVDH65 carrying the reporter gene β-glucuronidase intron (GUS-INT) and the marker ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Plant Biochemistry & Physiology
سال: 2016
ISSN: 2329-9029
DOI: 10.4172/2329-9029.1000167